Endophytic Trichoderma Species Isolated from Persea americana and Cinnamomum verum Roots Reduce Symptoms Caused by Phytophthora cinnamomi in Avocado.
Identifieur interne : 000221 ( Main/Exploration ); précédent : 000220; suivant : 000222Endophytic Trichoderma Species Isolated from Persea americana and Cinnamomum verum Roots Reduce Symptoms Caused by Phytophthora cinnamomi in Avocado.
Auteurs : Petra Andrade-Hoyos [Mexique] ; Hilda Victoria Silva-Rojas [Mexique] ; Omar Romero-Arenas [Mexique]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2020.
Abstract
Avocado root rot caused by the oomycete Phytophthora cinnamomi is a severe disease that affects avocado production in Mexico and worldwide. The use of biological control agents such as Trichoderma species isolated from places where the disease is always present, represents an efficient alternative to reduce losses. Thus, the objective of this research was to evaluate the biocontrol ability of 10 endophytic Trichoderma spp. strains against P. cinnamomi tested both in vitro and in the greenhouse. The endophytic Trichoderma spp. were recovered from Persea americana and Cinnamomum verum roots, isolated and purified on potato-dextrose-agar medium. Ten strains were identified by phylogenetic reconstruction of the internal transcribed spacer region of rDNA sequences as T.asperellum (T-AS1, T-AS2, T-AS6, and T-AS7), T. harzianum (T-H3, T-H4, and T-H5), T. hamatum (T-A12), T. koningiopsis (T-K8 and T-K11), and P. cinnamomi (CPO-PCU). In vitro dual-culture assay, the percentage of inhibition of radial growth (PIRG) between Trichoderma spp. and P. cinnamomi strains was measured according to the Bell's scale. PIRG results indicated that T-AS2 reached the highest value of 78.32%, and T-H5 reached the lowest value of 38.66%. In the greenhouse, the infection was evaluated according to the percentage of disease incidence. Plants with the lowest incidence of dead by avocado root rot were those whose seedlings were inoculated with T-AS2 and T-AS7, resulting in only 5% death by root rot caused by P. cinnamomi. The disease incidence of seedlings with wilt symptoms and death decreased more than 50% in the presence of Trichoderma spp. Relying on the results, we conclude that T. asperellum and T. harzianum contribute to the biocontrol of soil-borne pathogenic oomycete P. cinnamomi.
DOI: 10.3390/plants9091220
PubMed: 32957543
PubMed Central: PMC7569818
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72960</wicri:noRegion></affiliation></author><author><name sortKey="Silva Rojas, Hilda Victoria" sort="Silva Rojas, Hilda Victoria" uniqKey="Silva Rojas H" first="Hilda Victoria" last="Silva-Rojas">Hilda Victoria Silva-Rojas</name><affiliation wicri:level="1"><nlm:affiliation>Producción de Semillas, Colegio de Postgraduados, Campus Montecillo, Km. 36.5 Carretera México-Texcoco, Estado de México 56230, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Producción de Semillas, Colegio de Postgraduados, Campus Montecillo, Km. 36.5 Carretera México-Texcoco, Estado de México 56230</wicri:regionArea><wicri:noRegion>Estado de México 56230</wicri:noRegion></affiliation></author><author><name sortKey="Romero Arenas, Omar" sort="Romero Arenas, Omar" uniqKey="Romero Arenas O" first="Omar" last="Romero-Arenas">Omar Romero-Arenas</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de 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type="wicri:Area/Main/Exploration">000057</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Endophytic <i>Trichoderma</i> Species Isolated from <i>Persea americana</i> and <i>Cinnamomum verum</i> Roots Reduce Symptoms Caused by <i>Phytophthora cinnamomi</i> in Avocado.</title><author><name sortKey="Andrade Hoyos, Petra" sort="Andrade Hoyos, Petra" uniqKey="Andrade Hoyos P" first="Petra" last="Andrade-Hoyos">Petra Andrade-Hoyos</name><affiliation wicri:level="1"><nlm:affiliation>Manejo Sostenible de Agroecosistemas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Manejo Sostenible de Agroecosistemas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960</wicri:regionArea><wicri:noRegion>Puebla 72960</wicri:noRegion></affiliation></author><author><name sortKey="Silva Rojas, Hilda Victoria" sort="Silva Rojas, Hilda Victoria" uniqKey="Silva Rojas H" first="Hilda Victoria" last="Silva-Rojas">Hilda Victoria Silva-Rojas</name><affiliation wicri:level="1"><nlm:affiliation>Producción de Semillas, Colegio de Postgraduados, Campus Montecillo, Km. 36.5 Carretera México-Texcoco, Estado de México 56230, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Producción de Semillas, Colegio de Postgraduados, Campus Montecillo, Km. 36.5 Carretera México-Texcoco, Estado de México 56230</wicri:regionArea><wicri:noRegion>Estado de México 56230</wicri:noRegion></affiliation></author><author><name sortKey="Romero Arenas, Omar" sort="Romero Arenas, Omar" uniqKey="Romero Arenas O" first="Omar" last="Romero-Arenas">Omar Romero-Arenas</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960</wicri:regionArea><wicri:noRegion>Puebla 72960</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Avocado root rot caused by the oomycete <i>Phytophthora cinnamomi</i> is a severe disease that affects avocado production in Mexico and worldwide. The use of biological control agents such as <i>Trichoderma</i> species isolated from places where the disease is always present, represents an efficient alternative to reduce losses. Thus, the objective of this research was to evaluate the biocontrol ability of 10 endophytic <i>Trichoderma</i> spp. strains against <i>P. cinnamomi</i> tested both in vitro and in the greenhouse. The endophytic <i>Trichoderma</i> spp. were recovered from <i>Persea americana</i> and <i>Cinnamomum verum</i> roots, isolated and purified on potato-dextrose-agar medium. Ten strains were identified by phylogenetic reconstruction of the internal transcribed spacer region of rDNA sequences as <i>T.</i><i>asperellum</i> (T-AS1, T-AS2, T-AS6, and T-AS7), <i>T. harzianum</i> (T-H3, T-H4, and T-H5), <i>T. hamatum</i> (T-A12), <i>T. koningiopsis</i> (T-K8 and T-K11), and <i>P. cinnamomi</i> (CPO-PCU). In vitro dual-culture assay, the percentage of inhibition of radial growth (PIRG) between <i>Trichoderma</i> spp. and <i>P. cinnamomi</i> strains was measured according to the Bell's scale. PIRG results indicated that T-AS2 reached the highest value of 78.32%, and T-H5 reached the lowest value of 38.66%. In the greenhouse, the infection was evaluated according to the percentage of disease incidence. Plants with the lowest incidence of dead by avocado root rot were those whose seedlings were inoculated with T-AS2 and T-AS7, resulting in only 5% death by root rot caused by <i>P. cinnamomi</i>. The disease incidence of seedlings with wilt symptoms and death decreased more than 50% in the presence of <i>Trichoderma</i> spp. Relying on the results, we conclude that <i>T. asperellum</i> and <i>T. harzianum</i> contribute to the biocontrol of soil-borne pathogenic oomycete <i>P. cinnamomi</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32957543</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>17</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Endophytic <i>Trichoderma</i> Species Isolated from <i>Persea americana</i> and <i>Cinnamomum verum</i> Roots Reduce Symptoms Caused by <i>Phytophthora cinnamomi</i> in Avocado.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1220</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants9091220</ELocationID><Abstract><AbstractText>Avocado root rot caused by the oomycete <i>Phytophthora cinnamomi</i> is a severe disease that affects avocado production in Mexico and worldwide. The use of biological control agents such as <i>Trichoderma</i> species isolated from places where the disease is always present, represents an efficient alternative to reduce losses. Thus, the objective of this research was to evaluate the biocontrol ability of 10 endophytic <i>Trichoderma</i> spp. strains against <i>P. cinnamomi</i> tested both in vitro and in the greenhouse. The endophytic <i>Trichoderma</i> spp. were recovered from <i>Persea americana</i> and <i>Cinnamomum verum</i> roots, isolated and purified on potato-dextrose-agar medium. Ten strains were identified by phylogenetic reconstruction of the internal transcribed spacer region of rDNA sequences as <i>T.</i><i>asperellum</i> (T-AS1, T-AS2, T-AS6, and T-AS7), <i>T. harzianum</i> (T-H3, T-H4, and T-H5), <i>T. hamatum</i> (T-A12), <i>T. koningiopsis</i> (T-K8 and T-K11), and <i>P. cinnamomi</i> (CPO-PCU). In vitro dual-culture assay, the percentage of inhibition of radial growth (PIRG) between <i>Trichoderma</i> spp. and <i>P. cinnamomi</i> strains was measured according to the Bell's scale. PIRG results indicated that T-AS2 reached the highest value of 78.32%, and T-H5 reached the lowest value of 38.66%. In the greenhouse, the infection was evaluated according to the percentage of disease incidence. Plants with the lowest incidence of dead by avocado root rot were those whose seedlings were inoculated with T-AS2 and T-AS7, resulting in only 5% death by root rot caused by <i>P. cinnamomi</i>. The disease incidence of seedlings with wilt symptoms and death decreased more than 50% in the presence of <i>Trichoderma</i> spp. Relying on the results, we conclude that <i>T. asperellum</i> and <i>T. harzianum</i> contribute to the biocontrol of soil-borne pathogenic oomycete <i>P. cinnamomi</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Andrade-Hoyos</LastName><ForeName>Petra</ForeName><Initials>P</Initials><Identifier Source="ORCID">0000-0002-0501-9566</Identifier><AffiliationInfo><Affiliation>Manejo Sostenible de Agroecosistemas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva-Rojas</LastName><ForeName>Hilda Victoria</ForeName><Initials>HV</Initials><AffiliationInfo><Affiliation>Producción de Semillas, Colegio de Postgraduados, Campus Montecillo, Km. 36.5 Carretera México-Texcoco, Estado de México 56230, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Romero-Arenas</LastName><ForeName>Omar</ForeName><Initials>O</Initials><Identifier Source="ORCID">0000-0003-0076-3609</Identifier><AffiliationInfo><Affiliation>Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960, Mexico.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CVU-168369</GrantID><Agency>Consejo Nacional de Ciencia y Tecnología</Agency><Country></Country></Grant><Grant><GrantID>ID: 100420500</GrantID><Agency>Secretaría de Educación Pública</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">antagonism</Keyword><Keyword MajorTopicYN="N">biocontrol</Keyword><Keyword MajorTopicYN="N">disease incidence</Keyword><Keyword MajorTopicYN="N">root rot</Keyword><Keyword MajorTopicYN="N">soil-borne</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate 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